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KMID : 0385219990090020001
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Korean Journal of Gerontology
1999 Volume.9 No. 2 p.1 ~ p.6
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Ginsenosides Activate catalase from Bovine liver
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Lee Byung-Ryung
Sun Hyun-Jung
Kwon Hyeok-Yil
Park Jin-Seo
Lee Gil-Soo
Cho Jin-Hak
Choi Soo-Young
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Abstract
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The activation of bovine liver catalase by ginsenosides from panax ginseng C.A. Meyer has been studied. Preincubation of the enzyme with total ginsenoside, Rb₁, and Rc ginsenosides, increased catalase activities in dose and time-dependent manner, There was a reproducible decrease in Km, in addition to an increase in Vmax, in response to increasing concentration of the ginsenoside Rb₁ fraction. Upon addition of the ginsenoside Rb₁to the enzyme, a decrease in fluorescence intensity was discernible, together with an increase in emission anisotropy. Judging from the anisotropy values, the ginsenoside is rapidly trapped by the protein matrix. Rb₁ginsenoside was administrated to rats and the rat livers were removed for the measurement of the changes of antioxidant enzyme activities. Among the antioxidant enzymes, only the catalase activities were significantly increased after ginsenoside treatment. Therefore, it is suggested that the ginsenosides may decrease the H₂O₂level by activation of catalase and the enzymatic activation might be due to the conformational change induced by interaction of ginsenoside to the enzyme.
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KEYWORD
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Antioxidant, enzymes, Catalase, H₂O₂, Stimulatory, effect, Ginsenoside
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